DNA Extraction and Purification
Bulldog Bio Inc FastGene Scriptase II ReadyMix (100 rxns), Random Hex-primers
How would you construct the ideal reverse transcriptase? It would have to make very long cDNAs at high yields. It would have to be able to work from very small amounts of input RNA. And of course, it would have to work fast. FastGene Scriptase II checks all three boxes, and it does it at a reasonable price. Here’s how. First, mutations are inserted into the RNase H domain of native MuLV‘s reverse transcriptase delivering a higher yield of full-length cDNAs. Next, changes are made to increase the enzymes natural thermal stability providing a more robust enzyme that doesn’t lose processing power, even at elevated temperatures. Finally, the reaction buffer composition is optimized for high speed production of cDNAs from incredibly low levels of input RNA. This unique combination of size, speed, and production makes FastGene Scriptase II the optimal choice for even the most complex applications requiring cDNAs, such as RT-qPCR and NGS.
Zymo Research Corporation ZymoBIOMICS 96 MagBead DNA/RNA Kit (4 x 96 Preps.)
The ZymoBIOMICS MagBead DNA/RNA kit provides a high-throughput, magnetic bead-based purification of both high-quality DNA and total RNA (including small/microRNAs) from the same starting sample. The provided DNA/RNA Shield inactivates infectious agents and is ideal for sample storage at ambient temperatures. The extraction method utilizes magnetic beads for DNA/RNA extraction without the use of phenol and is eluted into ≥50 µl of ZymoBIOMICS DNase/RNase-Free Water. DNA/RNA is ready for any downstream application including Next-Gen Sequencing, RT-qPCR, etc.
Thomas Scientific SpinSmart Plasmid Miniprep DNA Purification Columns Only wi
SpinSmart Plasmid Miniprep DNA Purification Columns Only wi
Biochain Institute Inc Express Cloning Checker Kit II, 100 rxn/PK
BioChain's Express Cloning Checker Kits provide highly efficient methods of identifying recombinant colonies after transformation eliminating time-consuming plasmid preparation. In the large-scale screen method, the partial colonies of E. coli bacteria can be directly transfered from the transformation plates into the solutions of the kits and immediately run on an agarose gel electrophoresis. The results will be known within 1-2 hours instead of the 2 days required in a conventional method of labor-intensive plasmid DNA miniprep or colony hybridization.
Magbio Genomics Inc HighPrep Plasmid DNA Kit (1920 preps)
Magbio's HighPrep™ Plasmid DNA kit is a magnetic beads based plasmid purification system for high throughput rapid isolation of high and low copy number plasmid DNA, BACs, PACs Cosmid and Fosmids. The system can be easily automated on many liquid handling workstation providing robustness and high quality plasmid DNA for subsequent downstream applications such as digestion and fluorescent DNA sequencing
Biochain Institute Inc Genomic DNA - Parkinson's Disease: Brain: Parietal Lobe, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Magbio Genomics Inc HighPrep Plasmid DNA Kit (384 preps)
Magbio's HighPrep™ Plasmid DNA kit is a magnetic beads based plasmid purification system for high throughput rapid isolation of high and low copy number plasmid DNA, BACs, PACs Cosmid and Fosmids. The system can be easily automated on many liquid handling workstation providing robustness and high quality plasmid DNA for subsequent downstream applications such as digestion and fluorescent DNA sequencing
Macherey-Nagel NucleoSpin Tissue, 250 preps
NucleoSpin Tissue (250) 250 preps for the isolation of genomic DNA from tissue - NucleoSpin Tissue Columns, Collection Tubes, buffers, Proteinase K
Norgen Biotek Corp Bacterial Genomic DNA Isolation Kit
This kit provides a convenient and rapid method to isolate total DNA from fresh, frozen and preserved stool samples, including those preserved using Norgen’s Stool Nucleic Acid Collection and Preservation Tubes (Cat. 45660). The universal protocol conveniently allows for the isolation of total genomic DNA from all the various microorganisms and host cells found in the stool sample simultaneously. The kit removes all traces of humic acids and other inhibitors using a combination of chemical and physical homogenization and lysis. A simple and rapid spin column procedure is then used to further purify the DNA with high yields and molecular weights of up to 50 kb plus. The purified DNA is of the highest quality and is fully compatible with all downstream applications such as PCR, qPCR, Sequencing and microarrays since all humic acid substances and other PCR inhibitors are removed during the isolation process.
Biochain Institute Inc Genomic DNA - Human Diabetic Diseased Tissue: Skin, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
CUBE BIOTECH HisCube Ni-INDIGO MagBead MINI Kit; 1 kit
Ready to use kit for His-tag protein purification kit, for 50x purifications. 1x10 ml INDIGO Agarose, 2x50ml Binding Buffer, 2x50ml Washing Buffer,1 MagBead separator, 50 tubes, 1 x 500mM DTT, 1x500mM EDTA + Protocols
Biochain Institute Inc Genomic DNA - Liver Cirrhosis: Rectum, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Genomic DNA - Human Adult Normal Tissue: Heart, from a single donor, 100 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.